What Primers Does the Core Provide?

Here are the five primers we provide free of charge, along with their sequences:

T7 5'-TAA-TAC-GAC-TCA-CTA-TAG-GG-3'
T3 5'-ATT-AAC-CCT-CAC-TAA-AGG-GA-3'
SP6 5'-ATT-TAG-GTG-ACA-CTA-TAG-3'
M13 Forward 5'-TGT-AAA-ACG-ACG-GCC-AGT-3'
M13 Reverse 5'-CAG-GAA-ACA-GCT-ATG-ACC-3'

 

We supply the above primers free of charge. If you want to use one of these to sequence your template, just check the appropriate box on the submission form. Other primers are the responsibility of the individual investigators. Please see the section on Primer Design for suggestions on choosing your own primers. Consult the Table of Concentrations when you are ready to submit the primer for sequencing.

If there are other primers you think we should consider stocking, please let us know. These should be commonly-needed primers, and there must be a readily available source of template DNA to use as positive control (preferably a commercial product).