How You'll Get Your Results Back
Your results come in two formats: the sequence file and the chromatogram.
- The Sequence File:
This is simply a text listing of the sequence obtained from your DNA. It is what's known as a "flat text file", aka an ASCII file.
You'll automatically receive the sequence file by email when we have completed the analysis. You can read this file using any standard word processor, or you can import the sequence into most DNA sequence analysis software packages. The file is a flat text ('ASCII') file.
PLEASE NOTE that a text file does not necessarily contain accurate sequence! If your sample is weak or blank, any or all of the bases reported could be completely wrong. There are many artifacts that can similarly compromise the accuracy of the basecalls. You MUST examine your chromatogram in order to assess the validity of your results!
- The Chromatogram:
Each sequence generates a four-color chromatogram showing the partially-processed data from which the machine determined the nucleotide sequence. You should download your chromatogram from our Data Server. From the Core's Home Page, click on DOWNLOAD RESULTS under the MY ACCOUNT menu. You will need your PI's login name and the lab password.
Make sure you read the directions provided there. Note especially that these files are kept on the server for only about 1-2 weeks after sequencing is done! (The exact time for which files are kept depends on how much space we have on that server.)
We again must emphasize that the sequence file is virtually *useless* unless you also examine the chromatogram file. You need to know which base calls are reliable, which are not, and why they are not. Without the chromatogram, you simply cannot trust the raw, textual sequence data.
Please note, however, that your own experimental needs dictate whether the sequencing results you receive are adequate. We cannot edit your sequence, since to do so would require knowledge of your needs.
Please read Interpretation of Sequencing Chromatograms for further explanation.