The Fragment Analysis service offers the AMPFLSTR IdentifilerPlus Assay for cell line identification.This assay does identify Human Genomic DNA for 15 tetranucleotide repeat loci and the amelogenin gender determination marker.Our 3730XL Genetic Analyzer uses five fluorescent dyes per lane permitting multiplexing of four microsatellites and a fifth dyefor an internal size standard to correct for variability betweenlanes.
Genotyping - Microsatellite
Suzanne Genik firstname.lastname@example.org 734-764-8067
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Raw data .fsa files are generated from the 96 well CE plate.
GeneMarker software from SoftGenetics is available for clients to check out to analyze fragment analysis data.
|How to Request||
Sample Submission: go to https://client-seqcore.brcf.med.umich.edu then choose ‘Submit Fragment Analysis Samples or CE plates’
CE Loading plates submitted by clients must contain a size standard in 12ul/well of Hi- Di Formamide plus 1 ul of sample diluted in water.Clients may choose the number of wells to fill with samples.All wells must have 12ul of a liquid in them (Hi-Di Formamide or water with no sample).
Between 1 to 3 days, depending on how much business Sanger sequencing is processing.
Contact the core to consult the compatibility of the fluorescentdyes used with your samples and calibration dyes for our 3730XL Genetic Analyzer Instrument .A proper dilution of PCR products in CE Loading plates isnecessary to prevent over loading of array capillaries with highfluorescent signal intensities and could contaminate the next plate run.It is important to Label your CE loading plates with the identification plate number assigned by our database on submission.
Clients must have an account set up with the DNA Sequencing Core prior to submission of samples. The clients can use any of our drop off locations listed on our web page: http://seqcore.brcf.med.umich.edu/.
Ce Plates received from outside the University of Michigan can be shipped to: University of Michigan, DNA Sequencing Core, NCRC Bld 14, Room 122, 2800 Plymouth Road, Ann Arbor, MI 48109-2800. Plates must be shipped on gel ice packs.
CE loading plates prepared and submitted by clients are required to use ABI Optical plates or Optical ABI compatible 96 well plates with a barcode.
Acceptable bar coded plates are: