Individually Numbered Samples on Plates

If you need to submit a sample set that has at least 24 templates but less than 96, you can put your samples in a 96-well plate. This will not only save you the trouble of labeling multiple tubes but also minimize the amount of labeling required without promoting possible mistakes during sequencing.

If you are planning on submitting a FULL plate, and particularly if you want the discount associated with a full 96-well plate, please see the instructions here.

Below you find guidelines and examples for submitting partial plates, both what to do and what not to do.

  • Enter your samples into the computer either using the tube or bulk submission procedure. Do not use the plate submission process; that is for full 96-well plates.
  • Plates with conical wells are the best ones to use. Do not use plates with flat-bottom wells; we will not accept these.
  • Do not place 2 or more sample sets on the same plate unless the sample ranges are contiguousand have the same user.
  • Do not mix the primer(s) in with the templates.
  • Your first sample should be in well A1.
  • Proceed filling the first row (e. g., from A1 through A12) before continuing on the next row.
  • Do not skip any wells.
  • Write the sample number range (e. g., 1104725-1104796) on the plate itself, not on the cover seal.
  • Organize your templates so that those which use the same primer(s) are adjacent to each other. Avoid complicated primer patterns that might induce us to make a pipetting error!

Some examples:

If you are using only 1 primer for the entire set:

    (Empty wells at the                   (Samples in vertical                (Second row is not

      end of each row.)                     columns instead of                  completely filled.)

                                                       in horizontal rows.)

If you are using 2 (or more) primers for each sample in the entire set, your plate should look something like this:                                       

If you have multiple instances of 2 (or more) primers filling entire row(s), use the following example:                                         

 

            Two primers used for entire row.                                                        Each row has a different set of two primers.

In fact, you can change primers before filling an entire row. The example below shows the primers changing every 8 wells:

 

If you have a set in which all the samples use the P1, but only some of the samples need a P2, please refer to the diagrams below.

The two images represent the usual top-down view of a plate (top) and an �exploded� view of the plate from an angle (bottom). In both images, all the wells above the thick solid line represent the wells that will receive the P1 primer. The wells that are above the dashed line represent the wells that also receive the P2 primer.

In the exploded diagram, the lowermost portion shows the plate with templates in the appropriate wells. The layers above the plate indicate which of these wells will receive the P1 and P2 primers. (Notice the respective thick solid and dashed front edges.) The arrows are merely an aid for showing the alignment of the layers.

Always place the templates that receive multiple primers at the beginning of your sample set.

 

 

(Remember, no template can have a P2 primer without having a P1 primer as well.)

You may have a set in which the templates are arranged in blocks that use different primers. Do not use the following arrangement:

 

 

Instead, place the primers on separate plates, making sure that each primer well corresponds to that of the appropriate well on the template plate: