DNA Sequencing

Using an Applied Biosystems 3730XL 96 caplillary sequencer , the Core provides high-quality, extremely cost-effective DNA sequencin of PCR products, plasmids, siRNA constructs, DNA framents, and large DNA templates including plasmids over 20kb, cosmids, lamda clones, P1 clones, BACs and bacterial genomic DNA.

• Introduction to DNA Sequencing at the Core
• About the ABI 3730xl
• Standard Sequencing FAQ

Using the Illumina Cluster Station and Genome Analyzer, the Core provides in excess of 1Gb per experiment of highly accurate sequences of whole genomes, regions, genes, tags, and RNA populations at ~1% the current cost of capillary-based methods.

• About the Genome Analyzer
• About the Cluster Station
• About the Paired-End Module
• About sample preparation
• Sample Submission
• Illumina GA II FAQ
• Related Links

The Genome Sequencer FLX Instrument is designed to sequence clonally amplified deoxyribonucleic acid (DNA) fragments, which are derived from double-stranded sample DNA of various origins. The instrument can sequence de novo or re-sequence an average of at least 100 million bases per sequencing Run.

• About GS FLX454 Instrument
• Related Links