Individually Numbered Samples on Plates
If you are about to submit a sample set that has at least 24 templates (but less than 96), you can put your samples in a 96-well plate. This will not only save you the trouble of labeling multiple tubes but also minimize the amount of labeling required without promoting possible mistakes during sequencing.
If you are planning on submitting a full plate, please see the instructions <here>.
Below you find guidelines and examples for submitting “partial plates,” both what to do and what not to do.
For all sample sets:
If you are using only 1 primer for the entire set:
(Empty wells at the (Samples in vertical (Second row is not
end of each row.) columns instead of completely filled.)
in horizontal rows.)
If you are using 2 (or more) primers for each sample in the entire set, your plate should look something like this:
If you have multiple instances of 2 (or more) primers filling entire row(s), use the following example:
Two primers used for entire row. Each row has a different set of two primers.
In fact, you can change primers before filling an entire row. The example below shows the primers changing every 8 wells:
If you have a set in which all the samples use, say P1, but only some of the samples use P2, please refer to the diagrams below.
The two images represent the usual top-down view of a plate (top) and an “exploded” view of the plate from an angle (bottom). In both images, all the wells above the thick solid line represent the wells that will receive the P1 primer. The wells that are above the dashed line represent the wells that also receive the P2 primer.
In the exploded diagram, the lowermost portion shows the plate with templates in the appropriate wells. The layers above the plate indicate which of these wells will receive the P1 and P2 primers. (Notice the respective thick solid and dashed front edges.) The arrows are merely an aid for showing the alignment of the layers.
Always place the templates that receive multiple primers at the beginning of your sample set.
(Remember, no template can have a P2 primer without having a P1 primer as well.)
You may have a set in which the templates are arranged in blocks that use different primers. Do not use the following arrangement:
Instead, place the primers on separate plates, making sure that each primer well corresponds to that of the appropriate well on the template plate: