ONLY describes situations where the chromatogram and the text file
agree MOST of the
time, yet disagree in the first few nucleotides, or for individual
If you see more than a few nucleotides difference between two supposedly-matching files (.Seq and .ab1), please contact the Core Director immediately. There's something quite out of the ordinary occurring!
Between early February and mid-March of 2001, there may be a few nucleotides difference between the sequence in your email (i.e. Seq files) and that found on the chromatogram. One of the two computers we use was misconfigured, such that any editing performed by the technician was applied only to the chromatogram. The .Seq file (and thus the text email) would remain as they were when they first came off the sequencer.
The data in all cases is your own data, and the differences between .Seq and chromat files were relatively minor.
Typically, a technician might edit a sequence in the following situations:
of these situations, edits performed by the technician would appear in
but not in the .seq (text) file. This means the data on the
chromatogram may be slightly
better, since the technician manually corrected