Platform:
Applied Biosystems 3730xl DNA Analyzer
Category:
Sequencing
Primary Contact:

Constance Esposito, Assistant Director of Core Operations esposito@umich.edu 734-763-8895

Typical Cost:

Pricing does not reflect discounts offered to affiliates of certain research units or of specific center grants. Special project pricing may be available upon request.

Sample Type UofM Rates per well External Non-Profit Rates per well
Regular $3.00 $3.87
siRNA/Large Constructs $6.00 $7.74
96-Well Plate $2.50 $3.23
Ruch (Next Day) $4.50 N/A

 

Typical Output:

One sequencing run (one template, one primer) typically produces 900 nucleotides of high-quality sequence data or more, for a good quality template and a well-designed primer.

How to request:

Login through https://client-seqcore.brcf.med.umich.edu and then choose “Sanger Sequencing” to submit samples.

Sample requirements:

For our recommended template and primer concentrations, please go to faq page for Sanger Sequencing and read FAQ #17.

Typical Turnaround:

Samples are usually processed within two business days after receipt.

Critical information:

The results are returned in two forms: a raw sequence file and a chromatogram file. The sequence file (a flat ASCII text file) is returned to the researcher by electronic mail. The chromatogram file is available on our data server (password protected to prevent unauthorized distribution of your data).

More information:

Chromatograms and text files are available for download for 2 weeks after sequencing has been completed from our FTP server, after which the data is auto-deleted. The DNA Sequencing Core keeps all old sequencing data in archival storage, and can retrieve the data upon request by putting it on our FTP server. It will however be auto-deleted after 2 weeks. For more information on Sanger Sequencing please go to the FAQ page.

Platform:
Illumina HiSeq Libraries
Category:
Exome
Primary Contact: Typical Cost:

See the price lists.

Typical Output:

Illumina ready library

How to request:

Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’

Sample requirements:

1ug of good quality genomic DNA

Typical Turnaround:

4 weeks

Critical information:

We only stock the human kit. If you are looking for exome analysis of another species please contact us.

Platform:
Illumina HiSeq Libraries
Category:
RRBS
Primary Contact: Typical Cost:
Internal

$135 per sample + $19 per sample to multiplex

External

$174.15 per sample + $24.51 per sample to multiplex

 

Typical Output:

Illumina ready library

How to request:

Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’

Sample requirements:

1 to 3 ug of good quality DNA in 25-50ul

Typical Turnaround:

3 weeks.

Critical information:

Libraries will have slightly lower diversity.

Platform:
Illumina HiSeq Libraries
Category:
ChIP Seq
Brief Description:
Chromatin Immunoprecipitated DNA is prepared for sequencing on the Illumina platform.
Primary Contact: Typical Cost:

Internal

$144 per sample + $19 per sample for multiplexing

External

$185.76 per sample + $24.51 per sample to multiplex

Typical Output:

Illumina ready library.

How to request:

Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’

Sample requirements:

10-500ng of ChIP enriched DNA, up to 550bp

Typical Turnaround:

3 weeks

Critical information:
  1. input DNA is the appropriate comparison to ChIPped DNA, not an IgG control pulldown;
  2. a reference NON-pulldown gene is essential; and
  3. we have doubts about samples that don't show a 10-fold enrichment by qPCR performed as a delta-delta Ct
More information:

Illumina sequencing has size limitations due to the bridge amplification in cluster generation. We will not be able to sequence fragments larger than 550 bp.