Using the Illumina Methylation450 DNA Analysis kit, the DNA Sequencing Core performs high throughput sample Methylation profiling for intact and FFPE bisulfite converted DNA samples. The Core Services for the Illumina BeadArray platform consist of the following: 1). perform InfiniumHD Methylation assay, 2). Record signal intensities with an iScan Scanner, 3). create a GenomeStudio Project for data analysis, and 4). generate reports (Internal controls, SamplesTable, SampleMethylationProfile, GroupMethylationProfile).
Susan Dagenais, Ph.D.
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Methylation profiling of >450,000 loci. For generating Methylation Profiles, the iScan is set to record data that can be used with the Illumna GenomeStudio data analysis software as well as other software such packages such as Bioconductor.
|How to Request||
Sample Submission – Contact Susan Dagenais (firstname.lastname@example.org) and request a Project Quote. Please indicate the number of samples to be assayed and which Illumina BeadChip kit you would like used for the assay. We will e-mail you a Quote for the Illumina Kit costs and estimated Core Processing fees. Once we a signed Quote, we will place an order with Ilumina, assign a Core Project Number, and you will be sent a BeadArray submission form for sample submission.
The client will submit bi-sulfite converted DNA (8-10ul at 50ng/ul). Bi-sulfite converted DNA should be created using the Zymo EZ_DNA_Methylation kit (ZymoResearch) using Illumina incubation conditions (Appendix 6). For FFPE samples, we perform the Illumina FFPE Restoration protocol on bi-sulfite converted DNA submitted by the client.
Typical turnaround time is 1-3 weeks.