PyroMark SNP/Allele Quantification Analysis

PyroMark provides real-time sequence information, highly suitable for genetic analysis. It can be used to determine sequence variation--including single nucleotide polymorphisms and insertions/deletions--generating quantifiable results.

 

PyroMark SNP/Allele Quantification Analysis

Platform
PyroMark Q96 MD and PyroMark Q96 Vacuum Workstation
Category
Genotyping
Primary Contact

Ellen Pedersen

Research Specialist: Sequenom and PyroMark Services

elleped@umich.edu

University of Michigan DNA Sequencing Core - NCRC Building 14 Room 134

734-615-5646

Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical Output

Plate Report (text or Excel file) containing allele quantification percentages and sample/well quality metrics, along with Pyrograms depicting spectra for each well/sample.

How to Request

Contact Dr. Lyons for initial consultation; if he deems project appropriate for PyroMark, contact Ellen Pedersen.

Sample Requirements

Sample prep according to Qiagen’s specifications; Ellen will arrange direct support from Qiagen to ensure optimization of PCR products prior to sample submission for pyrosequencing.

Typical Turnaround

Depends on project queue; each plate/run requires ~4 hours to process.

More Information

If Assay Design is completed by Core, the costs will involve additional labor charges billed at the current aprroved PyroMark Tech time hourly rate, which will be added to the current per plate recharge rate.