Ultra low input gDNA

Ultra low genomic DNA (Thruplex Rubicon/ Low input Library prep Clontech)

Preparation of DNA for sequencing on the Illumina HiSeq. Described here is a library preparation service only. Once the library is prepared, you will also need to request (and pay for) the sequencing of that library.

DNA is fragmented, end repaired, A tailed and and the library is amplified by PCR with the Illumina Indexing Primer set.

 

Ultra low input gDNA

Platform
Illumina HiSeq Libraries
Category
Genome Sequencing (and gene expression profiling if used in conjunction with SMARTer kit)
Primary Contact
Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical Output

The Rubicon ThruPlex/Clontech Low input library prep kit will yield enough library to sequence on an Illumina HiSeq

How to Request

Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’

Sample Requirements

The total fraction of 50pg – 50ng full length, of fragmented double strand DNA.

 

Typical Turnaround

3 weeks

Critical Information

DNA is sheared by sonication on the Covaris. Sheared DNA is end repaired adapter ligaed and PCR amplified.