Sequenom iPLEX SNP Genotyping

The Sequenom® platform utilizes MALDI-TOF mass spectrometry in a cost-effective method that assays tens to hundreds of SNPs/indels in hundreds to thousands of samples, with multi-plexing capabilities as high as 40 targets per reaction.

 

Sequenom iPLEX SNP Genotyping

Platform
MassARRAY® (Sequenom, dba Agena Bioscience)
Category
Genotyping
Primary Contact

Ellen Pedersen Research Specialist: Sequenom and PyroMark Services University of Michigan DNA Sequencing Core NCRC Building 14 Room 134 734-615-5646

Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical Output

Excel files containing allele calls—separate file for each assay, delivered via email.

How to Request

Contact Dr. Lyons for initial consultation; if he deems project appropriate for Sequenom, contact Ellen Pedersen.

Sample Requirements

For each SNP-plex reaction, we need 5-10uL of 50-100ng/uL genomic DNA if quantitated on nanodrop, 25-50ng/uL if quantitated with picogreen on fluorimeter.

Typical Turnaround

Depends on number of samples and number of targets to be assayed.

Critical Information

The fixed costs described above do not include labor, which is charged at $66/hour and estimated as follows:

  • Assay design—2 to 4 hours, depending on number of targets
  • Sample processing—4 to 6 hours, depending on number of samples/SNPs
  • Reaction spotting—2 to 4 hours, depending on total number of chips
  • Data analysis—2 to 4 hours per chip
More Information

All custom primer orders are billed to a short code provided by the client: e.g., the interrogation of 40 variants requires 3 primers per target = 120 primers @ ~$5 per primer = ~$600. This order would yield enough primer to investigate at least 1,000 samples.