Sequenom EpiTYPEr - Methylation detection

The Sequenom® platform utilizes MALDI-TOF mass spectrometry in a cost-effective method that provides a highly scalable means of investigating a few or several hundred methylation regions over multiple samples.

 

Sequenom EpiTYPEr - Methylation detection

Platform
MassARRAY® (Sequenom, dba Agena Bioscience)
Category
Methylation Analysis
Primary Contact

Ellen Pedersen - Research Specialist: Sequenom and PyroMark Services
University of Michigan DNA Sequencing Core - NCRC Building 14 Room 134
734-615-5646

Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical Output

EpiReport Excel files containing tabulated methylation percentages and run metrics, and EpiGram jpeg files containing graphical representations of methylation levels.

How to Request

Contact Dr. Lyons for initial consultation; if he deems project appropriate for Sequenom, contact Ellen Pedersen.

Sample Requirements

Clients submit bisulfite converted and amplified products; these should be gel quantitated, according to the guidelines provided in the conversion and amplification kits recommended by Sequenom/Agena.

Typical Turnaround

Depends on number of samples and number of amplicons to be assayed.

Critical Information

The fixed costs described above do not include labor, which is charged at $66/hour and estimated as follows:

  • Sample processing—2 to 3 hours, depending on # of samples/amplicons
  • Reaction spotting—2 to 4 hours, depending on total number of chips