Sanger Sequencing

Sequencing of Plasmids (mini-preps, maxi-preps, 4-15kb); PCR products; Large DNA (plasmids over ~20 kb, cosmids, lambda clones); Large DNA (P1 clones, BACs, bacterial genomic); siRNA Constructs; Bacterial Genomic DNA; Rolling- Circle preps (e.g. 'TempliPHI' or  equivalent); Single Stranded (e.g. M13); DNA fragments (e.g. gel isolated fragments).

 

Sanger Sequencing

Platform
Applied Biosystems 3730xl DNA Analyzer
Category
Sequencing
Primary Contact

Constance Esposito, Assistant Director of Core Operations esposito@umich.edu 734-763-8895

Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical Output

One sequencing run (one template, one primer) typically produces 900 nucleotides of high-quality sequence data or more, for a good quality template and a well-designed primer.

How to Request

Login through https://client-seqcore.brcf.med.umich.edu and then choose “Sanger Sequencing” to submit samples.

Sample Requirements

For our recommended template and primer concentrations, please go to faq page for Sanger Sequencing and read FAQ #17.

Typical Turnaround

Samples are usually processed within two business days after receipt.

Critical Information

The results are returned in two forms: a raw sequence file and a chromatogram file. The sequence file (a flat ASCII text file) is returned to the researcher by electronic mail. The chromatogram file is available on our data server (password protected to prevent unauthorized distribution of your data).

More Information

Chromatograms and text files are available for download for 2 weeks after sequencing has been completed from our FTP server, after which the data is auto-deleted. The DNA Sequencing Core keeps all old sequencing data in archival storage, and can retrieve the data upon request by putting it on our FTP server. It will however be auto-deleted after 2 weeks. For more information on Sanger Sequencing please go to the FAQ page.