Rapid Paired end 100bp Sequencing

Rapid mode paired end sequencing on the HiSeq 2500 that produces paired 100 bp reads.

 

Rapid Paired end 100bp Sequencing

Platform
Illumina HiSeq Sequencing
Category
Rapid Paired end 100bp Sequencing
Primary Contact
Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical Output

27.5 Gb per lane

How to Request

Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’​​

Sample Requirements

30 ul of 10nM Illumina library with an insert size of at least 350 bp

Typical Turnaround

The run itself takes 27 hours the flow cell needs to be full for us to run it. Each flow cell has two lanes and we need to have a rapid mode HiSeq available. This could take some time to fill and run.

Critical Information

Rapid modes come at a premium while they are much faster run times they produce about 80% of the data as compared to a high output run.