NextGen Bisulfite Sequencing

Detection and quantification of methylation. Methylated DNA is treated with bisulfite which converts unmethylated cytosines to uracil. Methylated cytosines remain unchanged. This service yields the bisulfite-converted genomic libraries, but sequencing is an extra expense. Please consult with your bioinformaticist to determine appropriate parameters for sequencing, and required depth of sequencing.

 

NextGen Bisulfite Sequencing

Platform
Illumina HiSeq Libraries
Category
RRBS
Primary Contact
Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical Output

Illumina ready library

How to Request

Sample Submission: login through https://client-seqcore.brcf.med.umich.edu/ then choose ‘Illumina Sequencing’

Sample Requirements

1 to 3 ug of good quality DNA in 25-50ul

Typical Turnaround

3 weeks.

Critical Information

Libraries will have slightly lower diversity.