Microsatellite Genotyping - CODIS

Our Fragment Analysis service provides clients the ability tosize their DNA fragments using a fluorescence based detectionsystem on our 3730XL  Genetic Analyzer.A maximum of five fluorescent dyes per lane permitsmultiplexing of four microsatellites and a fifth dye for aninternal size standard to correct for variability between lanes.

 

Microsatellite Genotyping - CODIS

Platform
Fragment Analysis
Category
Genotyping - Microsatellite
Primary Contact

Suzanne Genik genik@umich.edu 734-764-8067

Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical Output

Raw data .fsa files are generated from the 96 well CE plate.

GeneMarker software from SoftGenetics is available for clients to check out to analyze fragment analysis data.

How to Request

Sample Submission: go to https://client-seqcore.brcf.med.umich.edu then choose ‘Submit Fragment Analysis Samples or CE plates’

Sample Requirements

CE Loading plates submitted by clients must contain a size standard in 12ul/well of Hi- Di Formamide plus 1 ul of sample diluted in water.Clients may choose the number of wells to fill with samples.All wells must have 12ul of a liquid in them (Hi-Di Formamide or water with no sample).

Typical Turnaround

Between 1 to 3 days, depending on how much business Sanger sequencing is processing.

Critical Information

Contact the core to consult the compatibility of the fluorescentdyes used with your samples and calibration dyes for our 3730XL Genetic  Analyzer Instrument .A proper dilution of PCR products in CE Loading plates isnecessary to prevent over loading of array capillaries with highfluorescent signal intensities and could contaminate the next plate run.It is important to Label your CE loading plates with the identification plate number assigned by our database on submission.

More Information

Clients must have an account set up with the DNA Sequencing Core prior to submission of samples. The clients can use any of our drop off locations listed on our web page: http://seqcore.brcf.med.umich.edu/.

Ce Plates received from outside the University of Michigan can be shipped to: University of Michigan, DNA Sequencing Core, NCRC Bld 14, Room 122, 2800 Plymouth Road, Ann Arbor, MI 48109-2800. Plates must be shipped on gel ice packs.

CE loading plates prepared and submitted by clients are required to use ABI Optical plates or Optical ABI compatible 96 well plates with a barcode.

Acceptable bar coded plates are:

  • 96 Well Optical Reaction Plates with Barcode code 128 from Life Technologies-AppliedBiosystems Part Number 4306737
  • 96 Well Semi-Skirted plate w/Raised rim, Barcoded from BioExpress Part Number T-3107-1BCCS
  • Hi-Di Formamide from Life Technologies-AppliedBiosystems Part Number 4311320C.