Kickin’ the tires

We at the DNA Sequencing Core sometimes get the opportunity to give a new instrument a try before committing to a purchase.  Right now at the Core in the Illumina Sequencing group we are kickin’ the tires on a few pieces of equipment:

  • HTG Molecular’s EdgeSeq
  • Agilent’s TapeStation 4200
  • Illumina’s NeoPrep

HTG Molecular’s EdgeSeq

HTG's extraction free (no nucleic acid extraction!) chemistry allows us to make next generation sequencing libraries directly from cell lines, fixed or frozen tissue, plasma, serum, and PAXgene.  They have targeted panels for miRNA and messenger RNA (including a systems biology panel with 2,560 genes, an immuno-oncology panel, and a lymphoma panel). The EdgeSeq is on campus and available for projects. We will keep the demo unit until late May 2016. For more information please visit HTG’s website: https://www.htgmolecular.com/

If you have more questions or are interested in bringing a project to us during the demo period, please contact Jeanne Geskes at jgeskes@umich.edu or our local HTG representative Scott Wernersbach at swernersbach@htgmolecular.com

Agilent’s TapeStation 4200

With 2 Agilent Bioanalyzers and one TapeStation 2200 already pillars of the DNA SeqCore’s RNA and DNA QC, the 4200 offers the fastest throughput with the least hands on time. We have the demo unit here for a couple of weeks. It allows us to load 96 samples at a time and walk away. For more information please visit Agilent’s website: http://www.genomics.agilent.com/en/TapeStation-System/4200-TapeStation-I...

If you have more questions or are interested in the 4200, please contact Jeanne Geskes at jgeskes@umich.edu or our local Agilent representative Pakito Martinez at pakito.martinez@agilent.com

Illumina’s NeoPrep

Illumina’s NeoPrep is a next generation sequencing library prep robot.  Using digital microfluidics the NeoPrep offers a simultaneous library prep of 16 samples with very little hands on time. The DNA kit requires 100ng but offers on board hands free library prep, PCR, quantification and normalization. The RNA library prep requires as little as 25 ng. We have the demo unit for another few weeks. For more information please visit Illumina’s web site: http://www.illumina.com/systems/neoprep-library-system.html

If you have more questions or are interested in the NeoPrep, please contact Jeanne Geskes at jgeskes@umich.edu or our local Illumina representative Scott Evans at sevans@illumina.com.