A Brief Introduction to the UM DNA Sequencing Core

General Overview : The University of Michigan DNA Sequencing Core provides nucleic acid analytical tools for the use of UM researchers and, in some situations, for researchers outside the UM as well. We occupy 10,000 sq ft of laboratory space in UM’s North Campus Research Complex (Building 14), with 30 staff members and an estimated $12M worth of capital equipment. The latter includes Sanger sequencers, NextGen sequencers (various types), microarray equipment, epigenomics analysis equipment and DNA/RNA quality control/quality assessment equipment.

This page provides only a superficial overview of the services. For additional information, please refer to our web site:

http://seqcore.brcf.med.umich.edu

Services : This Core provides analyses that would be difficult to carry out in individual labs, either due to the expense of the required equipment, or due to the level of expertise required to perform the analysis. Specific examples include:

  • Sanger sequencing

  • Next Generation sequencing

    • Illumina HiSeq

    • Ion Torrent/Ion Proton

    • Pacific Biosciences

  • Microarray analysis

    • Illumina microarrays (SNP typing, gene expression)

    • Affymetrix microarrays (SNP typing, gene expression)

  • Sequenom SNP typing

  • Qiagen Pyromark epigenetic analysis

  • DNA isolation from blood or saliva

  • DNA/RNA quality testing via Agilent Bioanalyzer or equiv.

  • Fragment sizing (e.g. CODIS genotyping, AFLP)

  • qPCR instrument access

To explore these service offerings further, please go to the Core’s web site (URL above) and peruse the menus for the relevant Frequently Asked Questions.

Support : The Core also offers a variety of support services to help our clients get and keep grant funding, design and troubleshoot experiments, and interpret the outcomes.

  1. Consultation: At no charge, we will work with our clients, helping them with experimental design. We can often help you choose a path among seemingly-similar analytical methods. In certain cases, we can provide data interpretation as well.

  2. Sample quality control: we have considerable resources and equipment for assessing the concentration and quality of your DNA or RNA samples.

  3. Letters of Support: When you write a grant proposal, ask us for a Letter of Support. We will help you demonstrate that you have the necessary resources and expertise to carry out a complex experiment involving any of our technologies.

  4. Site visits: If your Institute is site-visiting you, please allow us to help you, by demonstrating our commitment to technological excellence.

Questions

Our services are primarily intended to benefit the University of Michigan research community, but in some cases we may be able to accept samples from outside the UM. We do not perform testing for the lay public, i.e. we cannot provide paternity testing, ancestry testing, genetic testing for health-related issues or forensic testing to be used in court cases. Prospective clients must be associated with a reputable research institution.
Our services range in cost from $1 to tens of thousands of dollars. Please refer to the FAQ page specific to the service about which you are inquiring.
The various FAQ sections of our web site (see ‘FAQ’ menu, above) will provide details about individual categories of analysis services we provide. Alternatively, the ‘Platform’ and ‘Services’ menus will also provide a considerable amount of deeper discussion.
If you want to submit samples for analysis to this Core, please visit the Core’s FAQ pages. Look for the ‘General FAQs’ page, one of which gives instructions on “How to sign up as a new client”.