ChIP-Seq

Chromatin Immunoprecipitated DNA is prepared for sequencing on the Illumina platform. Described here is a library preparation service only. Once the library is prepared, you will also need to request (and pay for) the sequencing of that library.

 

ChIP-Seq

Platform
Illumina HiSeq Libraries
Category
ChIP Seq
Brief Description
Chromatin Immunoprecipitated DNA is prepared for sequencing on the Illumina platform.
Primary Contact
Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical Output

Illumina ready library.

How to Request
Sample Requirements

10-500ng of ChIP enriched DNA, up to 550bp

Typical Turnaround

3 weeks

Critical Information
  1. input DNA is the appropriate comparison to ChIPped DNA, not an IgG control pulldown;
  2. a reference NON-pulldown gene is essential; and
  3. we have doubts about samples that don't show a 10-fold enrichment by qPCR performed as a delta-delta Ct
More Information

Illumina sequencing has size limitations due to the bridge amplification in cluster generation. We will not be able to sequence fragments larger than 550 bp.