Affymetrix Microarray Gene Expression Analysis

Identification of differences in transcript abundance among 2 or more sources of RNA. mRNA analysis ranges from 3’end to whole transcript, including alternatively spliced variants. Additional products are available to assess miRNA.

 

Affymetrix Microarray Gene Expression Analysis

Platform
Affymetrix Microarray
Category
Gene Expression Analysis
Primary Contact

Christopher Krebs, Manager, UM DNA Sequencing Core

ckrebs@umich.edu

734-763-8891

Typical Cost Click here to view internal pricing list!
Click here to view external pricing list!
Typical Output

Over 40,000 RefSeq transcripts and over 10,000 lncRNAs are compared in a single gene expression experiment.

Over 30,000 mature miRNAs are compared with the latest miRNA arrays.

Data quality and group comparisons are assessed statistically and provided in a brief report. Raw data is exported as .CEL files and in an Excel spreadsheet. Help is available for submitting the data to external repositories (e.g., Gene Expression Omnibus (GEO) at NCBI).

How to Request

Login through https://client-seqcore.brcf.med.umich.edu and then choose “Sample QC” to submit samples.

Sample Requirements

A minimum of 250 ng total RNA for Standard Processing

A minimum of 10 ng total RNA for High Sensitivity Processing

Typical Turnaround

3 to 4 weeks, however, timing is dependent on availability of arrays, which are purchased as needed.

Critical Information

RNA quality and concentration are confirmed for an additional fee ($4.50 to $6, per sample) before beginning any Microarray project. Please consult with the Manager before submitting samples that are known or suspected to be of low concentration or highly degraded (i.e., from FFPE sources).

More Information

For a list of arrays available, product descriptions and additional technical information, go to the Affymetrix website at: http://www.affymetrix.com

For more information about RNA quality checks, go to: FAQ for QC

For other options for gene expression analysis (e.g., RNA-seq) go to: FAQ for Next Generation Sequencing (NGS).